Microsatellites are the target sequences of choice for a wide range of applications in genomics for genotyping,
genome mapping, QTL analysis and assessment of genetic diversity. We have effectively employed MetaPhor
agarose gel electrophoresis (MAGE) for genotyping with microsatellite markers. Separation of SSRs on MetaPhor
agarose gels appeared to be easier and economical. By employing MAGE, we were successful to clearly
separate SSR alleles with a size difference of five bp. MAGE is a reliable and appropriate approach for
identification of small length polymorphisms while screening large number of samples, therefore it is a valuable
tool for the researchers involved in DNA fingerprinting work with microsatellite markers.
Muhammad Asif, Mehboob-ur-Rahman, Javed Iqbal Mirza, Yusuf Zafar . (2008) High resolution metaphor agarose gel electrophoresis for genotyping with microsatellite markers, , Volume-45, Issue-1.
