Abstract
This study was undertaken at Livestock Experiment Station, Dept. of Livestock Management, University of
Agriculture, Faisalabad, Pakistan with an aim to determine the effect of post milking teat dipping +
Staphylococcus aureus vaccination on the frequency distribution of mastitogens in Sahiwal cows. For this
purpose, 20 lactating Sahiwal cows apparently free from mastitis were selected from livestock Experiment Station
herd where no mastitis control program was in practice. Treatments were C=control; D=Post milking teat dipping
only; V=Staphylococcus aureus vaccination only and DV=Teat dipping plus Staphylococcus vaccination. An
iodophore (Germ IODTM, Cenavisa S. A., Laboratories Distributed in Pakistan by Fair International Trading
Co.Karachi, Pakistan) was used as a teat dip @ 150ml /Iitre of water for a study period of 12 weeks.
Staphylococcus aureus vaccine prepared by Dept. of Clinical Medicine & Surgery University of Agriculture
Faisalabad. Pakistan was administrated I/m @ 5 ml/animal. Study results indicated that Staphylococcus aureus
(70%) was most prevalent followed by Streptococcus agalactiae (13.33%) and mixed infection (3.33%). However,
among minor pathogen, coagulase negative staphylococci (10%) were the most prevalent pathogens followed by
bacillus Spp. (3.33%) but none of the micrococci, diphtheroids and yeast were found. On overall basis, the
number of quarters affected by mastitogens decreased from 9 to 2 (77.7% reduction ), 8 to 6 (25% reduction) and
6 to 2 (66.6% reduction) due to teat dipping only, vaccination only and teat dipping plus vaccination, respectively.
However in control cows, number of quarter positive for intra mammary infection increased from 7 to 12 (71.10%
increase).
