Abstract
The study was conducted on lactating cattle and buffaloes in tehsil Burewala, district Vehari, Pakistan. The
purpose of study was to isolate different types of microorganisms associated with mastitis and to see the
effectiveness of different antibiotics against these isolates. Relative prevalence of Staphylococcus aureus was the
highest (50% and 53.85% in cattle and buffaloes respectively) followed by Streptococcus agalactiae (27.78% and
23.07%), Escherichia coli (16.67% and 15.38%), Streptococcus dysagalactiae (5.55% and 3.85%), and
Corynebacterium bovis (0% and 3.85%) in cattle and buffaloes respectively). On the whole, the isolates were
sensitive in descending order to Enrofloxacin, Chloramphenicol, Gentamycin, Oxytetracycline, and Amoxicillin.
