Different exotic dahlia cultivars namely Double Decorative and Double Giant were explored for shoot regeneration
using cotyledon leaf and hypocotyl explants on MS medium supplemented with combinations of plant growth
regulators (PGRs) viz. NAA and BA (A medium) and BA and IAA (B medium). Callus induction and shoot
regeneration was found directly proportional while root formation was inversely proportionate to the addition of
PGRs in the media. Maximum response for callus induction (60%) and shoot regeneration (13%) was found in
cotyledon leaf and hypocotyl explants, respectively, on medium containing 3 mgL-1 each of NAA and BA.
Significant genotypic variation was observed and cultivar Double Giant was found better for callus induction and
organogenesis at both media types. Indirect shoot regeneration could be helpful for mass propagation of elite
plant material and enhancing diversity in the ornamental plants.
B. Fatima, Muhammad Usman, T. Ashraf, R. Waseem, M.A. Ali. (2007) In Vitro Shoot Regeneration From Cotyledon And Hypocotyl Explants Of Dahlia Cultivars , , Volume-44, Issue-2.
