Abstract
The effects of liquid culture media, pH, temperature, and illumination on the
basidiospore germination of Lentinus swartzii and Lentinus strigosus as well as their
morphogenesis were studied and documented. Coconut water and potato broth with pH
of 5.0 – 7.0, incubated at 30°C and total dark, were the optimum nutritional and physical
factors in liquid culture condition as activators for successful basidiospore germination
of the two Lentinus species. A peculiar germination process was observed in which the
basidiospores germinated through swelling and elongated to become hyphae. The
vegetative phases of both mushrooms occurred in four significant stages: swelling and
clumping (a mechanism for plasmogamy), elongation, septation, and branching. On the
other hand, their reproductive phases had six distinct stages: mycelial coat formation,
browning and hardening stage (only in L. swartzii), popcorn-like formation (only in L.
strigosus), primordia initiation, pinhead stage, pileus expansion stage, and maturation
stage. This study established that the two Lentinus spp. have the same vegetative phase
(i.e., basidiospore germination and development of hypha and mycelia), but have
unique cultivation characteristics in their reproductive phase (i.e., formation of
basidiocarp). The obtained information on the developmental biology of the two
Lentinus spp. is very useful not only in the generation of effective mushroom biomass
production technologies but also in many fungal biotechnological applications.
Rich Milton R. Dulay, Esperanza C. Cabrera, Sofronio P. Kalaw, Renato G. Reyes, Ching T. Hou. (2020) Cultural conditions for basidiospore germination of Lentinus swartzii and Lentinus strigosus and their morphogenesis, Asian Journal of Agriculture and Biology, Volume 8, Issue-4.
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