Abstract
Polymerase Chain Reaction (PCR) detected concurrent infection of Clostridium (C.)
perfringens type D and C. chauvoei in samples of three cattle out of five which were
submitted to Veterinary Research Institute (VRI) for confirmation of C. chauvoei. The
animals had a history of fever, lameness and crepitating sound with death occurring
within 48 hours after the onset of clinical signs and seemed to be typical cases of black
leg. Furthermore, the traditional methods including clinical examination, necropsy
findings, cultural and biological methods are not solely enough for the confirmation of
disease and are not sufficient to determine the number of pathogens involved in such
cases.
Azam Ali Nasir, Muhammad Usman Ashraf, Asma Kausar, Nofil Mustafa, Zain-ul Fatima, Mobeen Sarwar, Rabia Riaz, Waseem Shahzad, Abdul Khaliq, Riaz Hussain. (2020) Detection of Clostridium perfringens Alpha, Epsilon and Clostridium chauvoei A toxin genes in Blackleg, Asian Journal of Agriculture and Biology, Volume 8, Issue-4.
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