Abstract
WRKY transcription factors group play a significant role in stress signalling pathway.
In this study, a WRKY member of bread wheat, TaWRKY, was cloned and its function
in response to water stress conditions was studied. Expression analysis revealed that
TaWRKY32 mainly expressed when plants were subjected to PEG, NaCl, and cold and
probably participate in ABA dependent signalling pathways. This study was done to
elucidate the role in transgenic breeding, TaWRK32 was transferred to rice plants under
CaMV-35S promoter. Overexpression of TaWRK32 lead to enhanced fresh shoot and
root weight at seedling stage when compared to wild type (WT). Moreover, transgenic
rice lines under water stress, showed greater recovery rate when compared to WT.
Higher values of cell membrane stability and lower Chl a/b ratio of transgenic rice lines
under water stress conditions markedly indicated the importance of TaWRKY32 under
water stress conditions. These results suggested that TaWRK32 has multifunctional and
might be an instrumental tool in breeding for transgenic plants for improved water
stress tolerance in wheat.
Essam Darwish, Shoaib Ur Rehman, Xinguo Mao, Ruilian Jing. (2021) A wheat stress induced WRKY transcription factor TaWRKY32 confers drought stress tolerance in Oryza sativa, Asian Journal of Agriculture and Biology, Volume-09, Issue-1.
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