Abstract
Background: Genetic diversity and population structure of the olive varieties cultivated in Pakistan are yet to
be explored.
Methods: In present study, we studied population structure and genetic diversity and developed DNA fingerprints
of 13 olive varieties using 63 Simple Sequence Repeat markers.
Results: Collectively 618 alleles were amplified among which 582 were polymorphic and 36 were monomorphic.
High allelic diversity per locus was found among 63 SSR markers, i.e., one for GAPU-12 to 23 for UDO099-008
and GAPU-47 with an average 9.80 alleles per locus. On the basis of cluster analysis genotypes were grouped
into two clusters. Cluster I contained varieties Manzanilla, Sohawa-selection, Koroneki, Bulkasar-selection,
Arbequina, Arbosona, Chugtai-selection, whereas cluster II was comprised of varieties Bari-Zatoon, Coratina,
Gemlik, Frontaio, Pendolino and Ottobratica. UDO-24 marker alone identified seven olive varieties. Similarly,
DCA-07 and EMO-02 identified six olive varieties each.
Conclusion: The findings of this manuscript will be helpful for future studies related to DNA fingerprinting and
genetic diversity assessment for choice of SSR markers and identification of olive varieties.
Muhammad Zaffar Iqbal, Shakra Jamil, Rahil Shahzad, Sajid Ur Rahman. (2020-2021) DNA Fingerprinting and Cultivar Identification of Olive (Olea europaea L.) using SSR markers, Advancements in Life Sciences, Volume-8, Issue-2.
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