Abstract
ABSTRACT: Multidrug resistant (MDR) and extensively drug resistant (XDR) E. coli strains generate diverse and
severe infections like bacteremia and urinary tract infections (UTIs) worldwide. They mostly carry antibiotic
resistance markers or genes on mobile plasmids making the treatment and eradication of such infections more
problematic. Plasmid eliminating agents (Ethidium bromide and Acridine orange) could be advantageous in the
expulsion of resistance bearing plasmids and eventually helpful in extermination of MDR and XDR-E. Coli.
Objective: This study was designed to determine the antibiotic resistance patterns of Escherichia coli isolates from
patients with urinary tract infections and bacteremia. We also detected the antibiotics resistance profiles and
compared the potential of curing agents in eliminating plasmid mediated antibiotic resistance.
Methods: Three hundred and fifty (350) E. coli isolates from patients diagnosed with urinary tract infections and
bacteremia were taken. Antibiotic sensitivity testing was accomplished by following CLSI (2015) protocol. Subminimum inhibitory concentrations (SICs) of Ethidium bromide and Acridine orange were determined by broth
dilution method in Luria Britani (LB) broth to find curing concentrations for resistance plasmids.
Results: E. coli which were (100%) resistant to Amoxicillin-clavulanate, Cefaclor, Cefuroxime, Cefixime,
Ceftazidime, Cefepime, Cefotaxime, Ceftriaxone, Ciprofloxacin and Pipidemic acid (urine only) and were selected
for curing analysis. About 46%, 16%, 14% and 14% of E. coli (blood isolates) were resistant to Cefoperazonesulbactam, Imipenem, Meropenem and Amikacin, respectively. About 34%, 13%, 12%, 10%, 9% and 6% of E. coli
from urine were resistant to Cefoperazone-sulbactam, Amikacin, Nitrofurantoin, Meropenem, Imipenem and
Fosfomycin, respectively. Sub-minimum inhibitory concentrations (SICs) of Ethidium bromide and Acridine
orange were effective between 125µg/ml to 1000µg/ml for both curing agent but the most impressive resistance
plasmid curing concentrations were 500-1000µg/ml and 500µg/ml of EthBr and AO, respectively. These both
curing agents were able to displace Imipenem, Meropenem, Cefoperazone-sulbactam, Nitrofurantoin,
Fosfomycin, Amikacin, Cefotaxime, Ceftazidime, Ceftriaxone, Ciprofloxacin and Cefepime resistance in E. coli.
The most prevailing eliminated resistance was of Imipenem and Meropenem. The study proposes that Ethidium
bromide and Acridine orange are pivotal in eradication of plasmid mediated antibiotic resistance in MDR and
XDR-E. coli.
Muhammad Akbar Hassan, Muhammad Salman Rasool, Fasihuddin Ahmed Ansari, Shahana Urooj Kazmi. (2020) Plasmid-Borne Drug Resistance Elimination Potential of Ethidium Bromide and Acridine Orange in Multidrug Resistant and Extensive Drug Resistant Escherichia Coli, International Journal of Pathology, Volume-18, Issue-2.
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