Abstract
Chemiluminescence (CL) in conjuction with flow injection analysis (FIA) shall be used to tide investigate the biotechnological potential of tris(2,2’-bipyridyl) ruthenium (II) system. CL using the Tris (2,2’-bipyridyl) ruthenium (II) system allows the development of methodologies for the analysis of nicotine amide adenine dinucleotide (NADH) and NAD-NADH converting biocatalyst(enzymes, dehydrogenase) and their substrates using immobilized biocatalyst. For example the analysis of lactate dehydrogenase and its isoenzyme is important for the diagnosis of myocardial; infarction. Certain hormones e.g. thyroxine inhibit glutamate dehydrogenases of Thyroxine and intern provide ways to treat goiter and thyroid cancer which is common in Pakistan. CL-FI manifold provide the ways to improve the sensitivioty,speed and cost effectiveness of clinical assays for hormones/drugs wittout using radioactivity.
