Abstract
Silver has been known for its antimicrobial activity for a very long time. The formulation of silver particles that range from 1-100nm in size makes it even more potent in terms ofinducingantimicrobial effects.Green chemistry has started toinfluencethe field of biochemical research. Silver Nanoparticles(Ag-NPs)synthesized through the green synthesis method provide a cheap and ecofriendlywayof nanoparticle preparation. Theaim of the current study is to prepare the green synthesis of Ag-NPsusing tomato juice as a reducing and capping agent and the evaluation of its antimicrobial activity. The stability and conformation ofAg-NPs was determined usingUV-visible spectroscopy. The antimicrobial activity of synthesizedAg-NPs was determined against E.coli DH5α.Ultraviolet spectroscopic analysis offered peak at 400 nm whichindicatedthe production of Ag-NPs of adequate size. E.coli DH5αdecreasedconsiderably upon the introduction of Ag-NPsto the bacterial inoculum. On increasing the concentration of Ag-NPs,an increase in the zone of inhibition was recorded. For 70μg/ml of Ag-NPs the zone of inhibition was 0.5 cm, while 0.6 cm, 0.7 cm and 0.7cm wererecorded for 100μg/ml, 150μg/ml and 200μg/ml of Ag-NPs,respectively. The efficacy of the antimicrobial activity of Ag-NPsderived from tomato juice proves its potential use in pharmaceutical and medicinal industries for the synthesis of nanomedicine.
Copyright (c) Mehreen Fatima, Azra Quraishi, Maida Akram
Mehreen Fatima, Azra Quraishi, Maida Akram. (2021) Natural Synthesis of Silver Nanoparticles from Grape Fruit Juice and Estimation of Antimicrobial Activity, BioScientific Review, Volume 3, Issue 2.
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