Abstract
Bacteria retain the ability to produce certain compounds to survive in unfavorable conditions; bioplastics is one of them. The aim of this study was to evaluate various environments for bioplastic producing bacteria. Bacterial strains were isolated from the desert and urban soil samples. A total of 39 strains isolated from Cholistan near Bahawalpur and 24 strains were isolated from various areas of Lahore. Bacterial strains were screened for the production and accumulation of bio-plastic. Only three strains from urban soil sample were able to produce
polyhydroxyalkanoates (PHA). Strain Acinetobacter seohaensis produced 40.6% bio-plastic when supplemented with coconut oil. This percentage is even higher than the glucose, which was 16.6 % after 24 h of incubation. The other competent strains i.e. Exiguobacterium indicum, Bacillus acidiceler were not able to utilize the coconut oil but produced 5 and 22% bio-plastic when supplemented with 2% glucose after 24 h of incubation, respectively. The percentage of the bioplastic produced when grown in nutrient broth was very low. The Fourier-transform infrared spectroscopy (FTIR) analysis of the extracted bio-plastic indicated the presence of the C=O functional group specific for the presence of polyhydroxyalkanoates, the absorbance peaks were
specifically at 1600 cm-1. Ribo-typing of all the six strains were carried out, strains identified as Exiguobacterium indicum, Bacillus acidiceler, Acinetobacter seohaensis, Serratia proteamaculans, Trabulsiella guamensis and Serratia grimesii and showed 98 to 100% similarity with sequences available in public domains.
Humaira Jabeen, Nazia Jamil. (2018) Responses of Bacteria from Urban and Desert Soil for Bioplastic Accumulation, Punjab University Journal of Zoology, Volume 33, Issue 1.
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