Abstract
Stevia is a natural sweet herb grown as a substitute for sugar producing crop. Its leaves have stevioside and have about 300 times more sweetness compared with other sugar producing crops. Its seeds are small and poor in germination hence can’t be used for mass propagation. Hence, this study was aimed to enhance propagation efficiency using Murashige and Skoog (MS) basal media supplemented with benzyl aminopurine (BAP), kinetin, indole acetic acid (IAA) and naphthalene acetic acid (NAA) in different combinations. Days to shoot induction (SI) were reduced to 4.6-5.6 days on M3 media (BAP + NAA) compared with M4 media (BAP and IAA) and mean shoot induction was greater on M3 media. Number of shoots induced per explant was higher (6 shoots/explant) on M1 medium containing higher levels of BAP and M3 media containing BAP and NAA (2.50 + 0.50 mgL-1 ). Shoot elongation, internodal distance, number of leaves, plant fresh weight, plant dry weight, and leaf fresh weight were higher at higher levels (2.50 + 0.50 mgL-1 ) of medium containing BAP + NAA (M3) and BAP + IAA (M4), respectively. Maximum root induction (83.33 %), a higher number of roots and more root length were found on M3 medium containing BAP and NAA (2.50 + 0.50 mgL-1 ). It is concluded that M3 medium containing BAP and NAA was better for enhanced shoot multiplication in stevia. The multiplied plant material was transferred to green house after acclimatization for further plant growth and biochemical studies. Such studies shall be useful for rapid clonal multiplication of the desired genotypes in stevia.
BILQUEES FATIMA, ZAHID NAZIR, MUHAMMAD USMAN, SYEDA ANUM MASOOD BOKHARI, SHUMAILA. (2021) Enhanced micropropagation in Stevia rebaudiana Bertoni, Biologia – Journal of Biological Society of Pakistan, Volume 67(I), Issue 1.
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