Abstract
Hepatocellular carcinoma (HCCa, also called malignant hepatoma) is a primary malignancy of the
liver. Most cases of HCCa are secondary to either a viral hepatitide infection (hepatitis B or C) or
cirrhosis (alcoholism) being the most common cause of hepatic cirrhosis. HCCa is the fifth most
common cancer and the third most common cause of cancer death worldwide. Many different proteins
are involved in HCCa including insulin growth factor (IGF) II , signal transducers and activators of
transcription (STAT) 3, STAT4, mothers against decapentaplegic homolog 4 (SMAD 4), fragile
histidine triad (FHIT), SIRT etc. The present study includes the bioinformatics analysis of SMAD 4
protein in order to understand the proteomic aspect and improvement of the diagnosis of the disease.
Different information related to proteins were gathered from different databases e.g. official Symbol
and chromosome location were taken from National Center for Biotechnology Information (NCBI)
Gene database. Structural weight (mass of the protein) was taken from Uniprot database. Length of
amino acids chain was taken from NCBI Protein database. Different domains of protein were taken
from InterProScan. The proteins details related to diseases were checked from Online Mendelian
Inheritance in Man (OMIM) database. The multiple sequence alignments of top eight closest
sequences to these proteins were performed by Claustalw2. Structure of the protein and evaluation of
the quality of the structures were included from Easy modeller and Pymol programs. This analysis not
only helped to gather the information related to the protein at one place but also analyzed the structure
and quality of the protein. Moreover, the present study also included the phylogenetic analysis among
different species based on the protein.
Muhammad Asif Rasheed, Sara Afzal, Fatima Tariq, Shazia Mannan. (2014) IN SILICO ANALYSIS OF SMAD4 INVOLVED IN HEPATOCELLULAR CARCINOMA, Asian Journal of Agriculture and Biology, Volume 2, Issue 1.
-
Views
915 -
Downloads
95
Article Details
Volume
Issue
Type
Language
