Abstract
Proximate analysis plays an important role in assessing the appropriateness of medicinal plants or their extracts orally taken by the marginal communities. It assessing its primary, secondary metabolites and to screened out in vitro biological assays of medicinally active plant. Quantitative analysis of Dicliptera bupleuroides was done by estimation of the primary and secondary metabolites (total carbohydrates, total starch, total proteins, total amino acids, total lipids, total glycosaponins, total alkaloids, total polyphenolics and total flavonoids) in plant powder. The maximum value of total carbohydrates (10.4 mg), total starch (4.81mg), total protein (15.78mg), total amino acids (7.87mg), total lipids (2.65mg)/100mL, total glycosaponins (11%), total alkaloids (5.51%), total polyphenolics (25.40%) and total flavonoids (3.58%) were respectively. Haemolytic, DNA protection assay and sun protection assay were determined according to the standard procedures. Different extracts showed various results however ethyl acetate fraction show best results in all assays while different fractions show diverse results. Results of the present study give an evidence for the existence of diverse primary and secondary metabolites and thus rationalizes its use in traditional medicines for the curement of different aliments owing to the safety profile on human red blood cells. There is a positive indication in safe use of this plant.
