STR markers, also known as microsatellites are dispersed across the whole genome of humans. STR markers are highly polymorphic due to different sort of mutations being carried out in genome. In forensics, genotyping of polymorphic STR markers lead to the solution of different issues such as paternity cases and identification of individuals. The present study aims in identification and determination of allele’s frequencies of four STR loci D5S818, D7S820, D13S317 and D16S539 in Pashtun population of Quetta Balochistan, Pakistan. Blood samples from 45 random individuals were collected after consent and were processed for DNA extraction. Amplification of STR markers by PCR followed by genotyping was performed. For marker D5S818, allele 147 bp was most common among all nine observed alleles with allele frequency of 37.77 % and allele 127 bp was least common with allele frequency of 6.66 %. For marker D7S820 most prevalent allele (62.22 %) was 212 bp among 6 observed alleles and alleles 200 bp and 208 bp were present in less individuals (11.11 %). Similarly,152 pb (66.66 %) was common allele among all individuals and allele 180 bp (11.11 %) was least common for marker D13S317. Out of five alleles observed for marker D16S530, allele 152 bp was commonly present (66.66 %) and allele 140 bp was least common allele (13.33%).
Palwasha Khan, Shakeela Daud, Asma Abro, Nida Ajmal, Saira Jamali. (2021) ESTIMATION OF ALLELE’S FREQUENCY OF FOUR STR MARKERS (D5S818, D7S820, D13S317 & D16S539) IN PASHTUN POPULATION IN BALOCHISTAN, PAKISTAN, , Volume 18, Issue 2.
