A number of plants contain various metabolites that have great medicinal importance. Green Biotechnology deals with the detection of those therapeutic compounds. Researchers focused on the isolation and mass production of these valuable compounds and to find the ways to use them in medicines in order to treat various disorders. In the present study, we used the ethanolic and methanolic extracts of Terminalia cattapa, Guaiacum officinale, Conocarpus erectus, Prosopis juliflora, Ziziphys nummularia, and Moringa oleifera to deterimine antimicrobial potential of these plants against pathogenic clinical isolates. Results showed that all the tested pathogens were inhibited by Terminalia cattapa; whereas Conocarpus erectus and Prosopis juliflora also showed antimicrobial activity against various pathogens. Guaiacum officinale only inhibited the growth of Escherichia coli while no inhibitory effect was observed by the extracts of Ziziphys nummularia and Moringa oleifera.