In this review, several conventional techniques have been described in order to purify monoclonal antibodies which include Bead-based chromatography, affinity chromatography with protein A to several alternative technologies including ion exchange chromatography, affinity chromatography and cation exchange chromatography. However, the demand of mABs in pharmaceutical industries is enhancing which requires high efficiency of the product which will render different polishing techniques to be encountered in such processes. These trends in advancement of the purification of mABs will lead to increased efficacy and fulfill the requirements of mABs in industry.
