Abstract
Pea (Pisumsativum L) is a major legume grown in Pakistan which used both as fresh vegetable and
canned food. However, pea production in Pakistan is under stress due to various abiotic and biotic
factors. Among biotic, the most destructive is bacterial blight caused by Pseudomonassyringae pv
pisi(Ppi). Therefore, in present study different pathogenicity assays were performed to observe the
innate immunity present in different commercial pea cultivars against Ppi isolated from pea seeds.
The results of pathogenicity assays showed that leaf detach method was best method for symptom
development as it took 2 days in comparison to foliar injection and soil drenching methods where
incubation period prolonged for 6-8 days respectively. Pea varieties viz. DMR-4, DMR-7 Green
forest, Rondo and Dasan showed susceptibility against Ppi isolates in all three methods of
inoculations. Commercially growing pea germplasm is not showing resistance against Ppi which
considered a looming threat to pea productions all over the world. Therefore, an extensive screening
of commercial pea germplasm against local bacterial isolates needed to be performed to avoid future
crop failure.
Muhammad Usman Raja, Muhammad Waseem Ali, Farid Asif Shaheen. (2016) DIFFERENT PATHOGENICITY ASSAYS TO EVALUATE VIRULENCE OF PSEUDOMONAS SYRINGAE PV.PISI ON COMMERCIAL PEA (PISUM SATIVUM L.) VARIETIES, Asian Journal of Agriculture and Biology, Volume 4, Issue 3.
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