Abstract
Extremophiles can thrive in extreme environmental conditions due to their unique and surprising adaptations towards harsh surroundings. In nature, these extremophiles may be capable to cope with two or more ecological stresses, hence called polyextremophiles. Halothermophiles is one of the important class of polyextremophiles, whose physiology is adapted to tolerate high temperature and salt concentration, therefore halothermophilic enzymes can be the classical tool in various biotechnology based industrial setups. Amylases (starch saccharifying enzyme) from halothermophiles have numerous applications in many industries. The purpose of current work is to isolate a novel halothermophilic bacterial strain from different extreme ecological regions i.e. salt mine, sea, hot spring etc. and to explore its ability to survive and produce amylolytic enzyme under raised temperatures and NaCl concentrations. For this, qualitative plate screening was done and enzyme index (EI) was calculated. According to results, the strain grew well and produced more amylase at 50˚C with 7 and 10% NaCl concentration and at 55˚C with 5% NaCl conc. That’s why it is suggested that this strain has great potential in industrial biotechnology
