Abstract
Citrus is the second largest fruit produced in the world and Pakistan is among the 12
large producers of the citrus fruit. It is grown in tropical and subtropical climate all over
the world. Besides its high economical & nutritional values citrus is attacked by
different pathogen such as fungi, bacteria, viruses and nematodes. Among all the
pathogens Colletotrichum gloeosporiodes causing citrus wither tip disease is one of the
major constrain in citrus production. Therefore current study was conducted to
investigate the different physiological characters on the mycelial growth of C.
gloeosporiodes and its chemical control. Among different fungal nutrient media Potato
Dextrose Agar (PDA) gave maximum mycelial growth (7.9 cm) followed by Citrus leaf
extract Agar (CLEA) (4.7 cm) and corn meal Agar (CMA) (3.3 cm).Temperature of
30°C favored maximum colony growth (8 cm) followed by 25°C (7.4 cm), 35°C (4.2
cm), 20°C (3.5 cm) and 15°C (2.3cm). A pH level of 6 favored maximum colony
growth (7.9 cm) followed by 5 (7.5 cm), 4.5 (5.3cm), 4 (4.8 cm), 7(3.3cm) and 3(2.4
cm).Among the application of different fungicides such as Topsin-M, Copper
oxychloride and Aliette at different concentration (300, 600 and 900 ppm). Topsin-M
gave maximum result to inhibit the Maximum mycelial growth inhibition of C.
gloeosporioides (1.1 cm) was produced by Topsin-M 9 days after incubation at 30 °C
as compared to control (7.6 cm).
Salman Ghuffar, Muhammad Zeshan Ahmed, Muhammad Farooq Aslam, Luqman Amrao, Sajjad Hyder. (2017) Physiological Studies on Colletotrichum Gloeosporiodes Associated With Wither Tip Disease of Citrus and Its Chemical Control, Asian Journal of Agriculture and Biology, Volume 5, Issue 2.
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