Momordica charantia L., a plant of the family cucurbitaceae, commonly used in Benin, is known for its antimicrobial potency. This research evaluates the biological properties of M. charantia for safe use. The material used consisted of eight bacterials, ethanolic extract of Momordica charantia and female wistar strain rats. Minimum inhibitory concentration (MIC) and Minimal bactericidal concentration (MBC) were investigated by microdilution and plating, kinetics of action by spectrophotometry, and reversion of bacterial resistance by microdilution. The Traces Metallic Elements (ETM) were assayed by Metalyser protocol. The safety tests were performed according to the Organisation for Economic Co-operation and Development (OECD), Standards 423 (Acute Toxicity) and 407 (Subchronic Toxicity) guidelines and the blood sample by retro-orbital sinus puncture. The significance of the statistical results was set at P ˂ 0.05%. The results reveal 90% of tested germs sensitive to the extract of M. charantia whose search for MIC and MBC shows a bactericidal effect on 57.14% and bacteriostatic on 42.86%. The kinetics of action reveals an antibacterial effect between 8 to 16 hours in contact with germs. The conventional antibiotic-extract combination reveals a significant change in MICs with decreases of up to 90%. Acute toxicity has not shown significant modification of the cellular structures on the histological sections. M. charantia has antibacterial properties and its punctual use is safe at the doses tested.
Houéto E.E.M., R.C. Johnson, A.M.O. Amoussa, H.W. Kpètèhoto, F.M.Z. Mignanwandé, F. Loko, L. Lagnika. (2019) ANTIMICROBIAL POTENCY AND REVERSION OF THE BACTERIAL RESISTANCE OF ETHANOLIC EXTRACT OF MOMORDICA CHARANTIA LINN., , Volume 16, Issue1.
