Abstract
This study reports antifungal activity of n-butanol fraction of methanolic leaf extract of Kochia indica Wight against Macrophomina phaseolina and its phyto-components profiling through GC-MS analysis. Eight concentrations of the extract, ranged from 1.562 to 200 mg mL-1 were prepared in liquid malt extract medium. Bioassays revealed the significant inhibitory activity of all the concentrations resulting in 63-92% reduction in biomass of M. phaseolina. GCMS analysis revealed the presence of 29 compounds in the fraction. Among these pentane, 2-butoxy- (8.55%); propane, 2,2-diethoxy- (8.21%); butane, 1-(1-ethoxyethoxy) (8.19%); 5-methyl-2-hexanol (7.48%) and 1-butanol, 3-methylacetate (7.36%) were the most abundantly occurring compounds, which may possibly be responsible for antifungal behavior of n-butanol fraction of methanolic leaf extract of K. indica against M. phaseolina.
