The current study was carried out to identify the key genes and their mechanism underlying the myositis. The integrated analysis of expression profiling. In silco was conducted to find the differentially expressed genes (DEGs) in IIM. Moreover, functional gene annotation, pathway enrichment analysis, construction of protein-protein interaction (PPI) network and identification of small molecule were applied for exploring the potential biological roles of DEGs in IIM. Overall 204 DEGs were found in IIM patients compared using healthy controls. The analysis of DEG resulted in alteration of different metabolic and regulatory processes which are distributed as 88 biological processes, 26 molecular functions and 33 cellular component using DAVID online tool. KEGG was used for pathway enrichment analysis for identified DEGs after applying P value (2) as threshold. Protein-protein interactions network was constructed and analyzed using STRING and Cytoscape tools and total 15 Hub proteins were identified with minimum 50 and maximum 93 degrees. Finally, Connectivity map (CMap) analysis was performed to identify 20 small molecules capable of altering the expression of gene in IIM. Study concluded the valuable information regarding the pathogenesis mechanism of myositis and suggesting a few compounds as a drug against IIM.
