• Babar Khan

• Nazir Javed

• Sajid Aleem Khan

• Nasir Ahmed Rajput

• Huma Abbas

• Abdul Jabbar

• Madassar Walait

• Zuniara Akash

Abstract

Entomopathogenic nematodes belong to family Steinernematidae and Heterorhabditidae. They are obligate parasites of different insect pests and are used as natural control agents. The aim of this work was to check the virulence of Steinernema glaseri against two insect larvae belongs to genus lepidoptra. Three hundred infective juveniles at 27±2ºC were used in each treatment. S. glaseri was more effective against armyworm as compared to pink bollworm larvae. Effects of different concentrations and storage time of S. glaseri were also evaluated on these larvae. At 500 IJs concentration high virulence was recorded followed by 200, 100 and 50 IJs at 27±2ºC. High virulence of S. glaseri was recorded in 2 weeks followed by 4 and 6 weeks old culture. Maximum population of S. glaseri was recorded on 0.5g as compared to 0.25g larval weight. These findings will hopefully be helpful for researchers and farmers for batter management against both insect pests.

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