Avian mycoplasmosis is caused by several pathogenic mycoplasmas species including Mycoplamsa gallisepticum, M. synoviae, M. meleagrides and M. iowae. M. gallisepticum (MG) and M. synoviae (MS) are responsible for considerable economic losses in the poultry industry, especially in Pakistan and other developing countries. Hence constant surveillance is required for effective control of avian mycoplasmosis in this region. In the present study serological (immunodiffusion, ELISA) and PCR tests were performed and their efficiency on healthy and experimentally and clinically infected chickens’ sera was evaluated. Isolation of pathogen from infected birds is a gold standard for disease diagnosis. During the present study, mycoplasma was isolated from only less than 10% clinically positive samples and among these isolates 73.63% were identified as M. gallisepticum by using specie specific PCR technique. ELISA was found to be the most sensitive serological test compared to immunodiffusion for detection of anti-M. gallisepticum antibodies in the sera of infected birds; which gave 88% sensitivity with 92% specificity. Among the clinically infected bird samples, 72% samples were found positive for anti- M. gallisepticum antibodies. Interestingly, 28.71% of clinically healthy birds' sera also showed the presence of antibodies, indicating the extent of mycoplasma burden on the poultry farms of this region.
Faiz Muhammad, Jehan Alam, Johar Hussain, Syed Khurram Fareed, Urooj Zafar, Shakeel Ahmed Khan, Aqeel Ahmad. (2018) DEVELOPMENT OF ELISA AND ITS COMPARISON WITH OTHER DIAGNOSTIC TESTS FOR AVIAN MYCOPLASMOSIS, , Volume 15, Issue 1.
