Abstract
In vitro study was conducted to evaluate the effect of fourteen selected pesticides
including six fungicides, four insecticides and four herbicides on the growth of a local
strain of Trichoderma harzianum and on possible antagonistic activity of the treated
fungus against Fusarium oxysporum. For compatibility study, each pesticide was
tested at seven concentrations using poisoned food technique. While for the
antagonistic activity study, treated T. harzianum was tested against F. oxysporium
using dual culture technique under laboratory conditions. Significant differences were
observed between the pesticides and the concentration used in the inhibition of
mycelial growth and the inhibition increased with concentration increase. None of the
concentrations tested of wettable sulphur, copper oxychloride, diazinon, cypermethrin,
oxamyl, tribenuron-methyl and metribuzin suppressed the mycelial growth and the
antagonistic potential of T. harzianum against F. oxysporium indicating the possibility
of the integration between these pesticides and T. harzianum without any fear.
However, the use of incompatible pesticides at all tested concentrations such as
penconazole, iprodione, fenarimol and mancozeb and high concentrations of
oxyfluorfen, glyphosate and imidacloprid may lead to inhibition of the growth and the
antagonistic activity of T. harzianum as well as caution must be taken when using these
pesticides in the disease management program. The obtained results will enable choice
of combining T. harzianum with the selected pesticides within integrated disease
management strategy
Nowara A. Mohamed, M. A. Radwan. (2017) Impact of pesticides on Trichoderma harzianum and on its possible antagonistic activity against Fusarium oxysporum under In vitro conditions, Asian Journal of Agriculture and Biology, Volume 5, Issue 4.
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