Biochar and compost were used to appraise their antifungal activity against Botryodiplodia theobromae Pat. causing die back disease in several cost-effective vital crops. Various concentration of aqueous and methanolic extract (1-3%) of biochar and compost were applied against the test fungus. Highest antifungal activity was shown by biochar extracts in contrast to compost extracts. Different organic fractions viz. n-hexane, chloroform, ethyl-acetate and n-butanol from biochar extracts were separated. Minimum Inhibitory Concentration (MIC) assay was also conducted with these isolated fractions by using serial dilution method. The MIC (10 - 0.019 mg mL-1 ) for each fraction was noted at 24, 48 and 72 hours intervals. n-butanol and Puslan (synthetic fungicide) were effectively inhibited the spore germination of B. theobromae with MIC of 0.019 mg mL-1 . This study concluded that biochar and compost possess antifungal potential against B. theobromae. So, they may be exploited for the isolation of nature friendly antifungal compounds.
