The aim of this study was to detect genetically modified (GM) contamination in random samples of IRRI-6 rice from Pakistan. DNA was extracted by modified cetyltrimethyl ammonium bromide (CTAB) method. The polymerase chain reaction (PCR) assay was conducted with three primers to amplify the 35S promoter of cauliflower mosaic virus (CaMV-35S), nontranslated region of the nopaline synthase (T-NOS) gene and neomycin-phosphotransferase II (NPTII) gene. Primer of sucrose phosphate synthase SPS gene was used as internal control of rice. SPS primer amplified specific single band while the other three primers failed to produce any band in any of the samples and confirmed that the analyzed commercial samples of IRRI-6 rice variety were free from GM contamination. Further, these primers (except SPS) used for genetically modified (GM) cotton to cross check; all primers showed amplification and produced band of specific size which confirm the presence of GM in cotton.
Huma Rao, Naheed Kauser, Ameer Ahmed Mirbahar, Syeda Kahkashan Kazmi, Saifullah Khan, Sajjad A. Rao. (2016) DETECTION OF GM CONTAMINATION IN IRRI-6 VARIETY OF RICE (ORYZA SATIVA L.) GROWN IN PAKISTAN, , Volume 13, Issue 4.
