Abstract
Herbicides are used for the eradication of weeds. 2,4-D is the post emergence herbicide, commonly used to kill unwanted plants. The present study was carried out to determine the effect of different concentrations of 2,4-D herbicide on stomatal characteristics of flag leaf of wheat (Triticum aestivum L.). The field experiment was conducted during the year 2013-14 and 2014-15. The experiment was laid out in a randomized complete block design (RCBD). At tillering stage of wheat, 2,4-D herbicide was sprayed and at milking stage wheat samples were collected to analysed the effect of 2,4-D on stomatal characteristics of flag leaf of wheat. High stomatal length of adaxial epidermis and abaxial epidermis was noted in plots treated with 0 M, 0.01 and 0.03 Mconcentrations of 2,4-D. Concentration 0.03 M showed highest stomatal width of adaxial epidermis and non-significant effect of different concentrations of 2,4-D herbicide was noted on stomatal width of abaxial epidermis. Maximum stomatal density of adaxial epidermis and abaxial epidermis was observed by application of 0.01 M and 0.03 M concentrations of 2,4-D herbicide. Concentration 0.04 M showed highest stomatal index of adaxial epidermis while 0.01 M and 0.03 Mconcentrations showed maximum stomatal index of abaxial epidermis.
