Abstract
In this paper we studied the isolation and identification of phytoplankton from aquatic
ecosystems of Lampung Mangroves Center (LMC) as biology feed. The analysis of
sea water quality parameters in the location of the study consisting of pH, salinity,
brightness, water temperature, nitrate, nitrite and phosphate, revealed that all the
parameters meet the quality standard, except for nitrates and nitrites. Isolation and
identification of Phytoplankton taken from aquatic ecosystem of the LMC using net
plankton ± 15 was carried out to obtain pure cultures in Live Feed Laboratory of the
Center for Marin Culture Lampung, Indonesia. The Phytoplankton samples were
isolated in the bacto agar and were added the fertilizers Conwy and Gillard to spur the
growth of green and brown algae. The isolation of media was done by scratch method
and cultured in a petri dish with upside down position for seven days, and observed
every day for evaluating the algae growth. The results showed that there were 14
genera of phytoplankton identified. Six types of 14 genera of phytoplankton
identified have potential to be developed as a biological feed: Thalassiosira,
Cyclotella, Chaetoceros, Tetraselmis, Nannochloropsis, and Isochrysis, The isolates
of phytoplankton produced can be stored in the refrigerator as pure stock cultures and
can last for up to 6 months before being used in a medium culture or laboratory culture
scale.
Tugiyono, Jani Master, Suharso. (2017) Isolation and identification of phytoplankton from aquatic ecosystems of Lampung Mangroves Center (LMC) as biological feed, Asian Journal of Agriculture and Biology, Volume 5, Issue 4.
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