Abstract
Background: Lithium organic compounds are considered safe in use as compare to inorganic compounds of lithium for the treatment of various psychiatric disorders. The aim of present study was to check the effect of lithium citrate on tripeptide master antioxidant glutathione of cytosolic fraction of human blood.
Material & Methods: It was an experimental study which was conducted in PhD research laboratory in Faculty of Pharmacy, Gomal University, D.I.Khan, Pakistan, from January 2013 to March 2014.Blood components were separated by centrifugation at 10000 – 12000 rpm for 10 minutes method. The supernatant pale yellow layer was cytosolic fraction or lysate. 50μl of 0.1N HCl solution was added to keep GSH in reduced form.
Results: The cytosolic fraction GSH level was decreased significantly (p<0.001) which was 3.739 μM (72.66%) by lowest used concentration of lithium citrate while the drop in GSH by other used concentrations of lithium citrate was 3.624 μM (70.42%), 3.567 μM (69.32%), 3.484 μM (67.70%), 3.420 μM (66.46) and 3.338 μM (64.87%) respectively.
Conclusion: Although organic compounds of lithium are said to be safer in use as compare to inorganic compounds of lithium but our study suggests that lithium citrate can considerably depletes GSH in cytosolic fraction of human blood.
Hashmat Ullah, Muhammad Farid Khan, Sattar Bukhsh Awan, Habibullah Khan, Farwa Hashmat, Ubedullah Shaikh, Muhammad Akhlaq. (2016) IMPACT OF DEPLETION OF ERYTHROCYTE CYTOSOLIC-FRACTION GLUTATHIONE BY ORGANIC LITHIUM COMPOUND ON IMMUNE SYSTEM, Gomal Journal of Medical Sciences , Volume 14, Issue 2.
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