The study examined the effect of UV-B radiation and allelochemical stress on germination, seedling growth, chlorophyll a and b content and amylase activity of Vigna radiata. The individual effect of UV-B radiation and aqueous extract of Pluchea lanceolata inhibited germination of all test species. The species showed differential response. Germination was reduced by both the UV-B radiation and aqueous extract of Pluchea lanceolata in order: Lens culinary >Vigna mungo >Vigna radiata. Combined stress showed decrease in germination as well as root length, shoot length8l. ;and fresh weight of Vigna radiata. The individual effect of UV-B radiation increased the chlorophyll a and b while the individual effect of aqueous extract decreased the chlorophyll content whereas combined stress of UVB and aqueous extract of Pluchea lanceolata also decreased the chlorophyll content in Vigna radiata. Simulated rain leachate of Pluchea lanceolata also inhibited the germination, root length and shoot length of Vigna radiata. Decaying Pluchea lanceolata in soil at a rate of 5, 10, 20gm/350gm soil substantially inhibited the germination but increased the shoot length and decreased the root length of Vigna radiata. UV-B radiation and aqueous extract of Pluchea lanceolata alone and in combination decreased the amylase activity of Vigna radiata.
Naila Waseem , S. Shahid Shaukat. (2016) EFFECT OF ENHANCED UV-B RADIATION, ALLELOPATHY AND THEIR COMBINED STRESS ON GERMINATION, SEEDLING GROWTH, PIGMENTS AND AMYLASE ACTIVITY IN VIGNA RADIATA, , Volume 13, Issue 3.
