Seed proteins are important in grain quality and distinguishing genetic diversity for breeding populations. The present study was performed to evaluate genotypic diversity in protein bands in common buckwheat based on SDS-PAGE from Skardu and Ganche Districts of Gilgit-Baltistan. Seed protein profiles among indigenous genotypes (12) were assessed for genetic diversity and cluster analysis. Buckwheat protein extracts were resolved on 12.25% acrylamide in discontinuous buffer system. Protein bands produced were produced were analyzed for cluster analysis by UPGMA revealed two lineages at linkage distance of 0.39 dividing the local genotypes into 5 sub cluster groups at 0.19 (50%) linkage distances. Protein bands on SDS-PAGE were scored from 15 kDa to 72 kDa producing 37.27% (HMW) and 62.72% LMW protein bands. The ratio of HMW protein bands to total bands produced were low (0.214) as compared to LMW protein bands (0.359). The protein profiling produced revealed moderate genetic diversity between studied genotypes and could be helpful for utilization in cultivar development and conserve buckwheat germplasm.
Ishtiaq Hussain, Asghari Bano, Faizan Ullah, Muhammad Ali, Sikandar Khan Sherwani. (2016) GENOTYPIC VARIATION AMONG INDIGENOUS COMMON BUCKWHEAT OF BALTISTAN BASED ON SDS-PAGE, , Volume 13, Issue 2.
