Abstract
Ten different genotypes of Brassica napus L. were screened for callus induction and regeneration capabilities. The effect of different growth regulators and carbon sources on callus induction and regeneration was investigated using seven different callus induction media (CIM) compositions (CIM-1 to CIM-7) and five different shoot induction media (SIM) compositions (SIM-1 to SIM-5) along with controls. Among the callus induction media compositions supplemented with 3% sucrose or glucose as a carbon source, CIM-7 (Murashige and Skoog (MS) media supplemented with 4 mgL -1 NAA, 0.4 mgL-1 BAP and 0.4 mg L-1 Kinetin) resulted in maximum callus induction i.e. 80% and 69%, respectively. Among different SIM media supplemented with 3% sucrose or glucose, SIM-1(MS media supplemented with 0.3 mgL-1 NAA and 3 mg L-1 BAP) gave maximum shoot induction i.e. 16% and 9%, respectively. On shoot induction Driver-Kuniyuki walnut (DKW) media with 3% sucrose, maximum shoot induction (10%) was observed on SIM-1 (DKW media supplemented with 0.3 mgL-1 NAA and 3 mg L-1 BAP). Among the ten genotypes tested, the advanced line NIFA Nr.2 resulted in maximum callus induction (76%) on CIM media with 3% sucrose, whereas, the advanced line NIFA Nr.6 gave maximum callus induction (67%) on CIM containing 3% glucose. Maximum shooting (12.963%) on DKW media was observed for NIFA Nr.1. On SIM with 3% sucrose and glucose, the same line showed maximum shooting i.e. 18% and 10%, respectively. The shoots obtained were then transferred to half strength MS media with or without growth regulators (control media) for root development. The well rooted plants were transferred to soil and successfully grown under green house. The above mentioned combinations and concentrations provide base line information for future tissue culture based Brassica improvement programs especially via transgenic interventions.
