Abstract
Ever increasing growth of food and biotechnological industries has built up a stress over researchers for the large investigation of microorganisms, which could be used in industries. In the present work cultural conditions for the production of Invertase from Mucor geophillus using agricultural wastes in submerged fermentation along with partial purification and characterization were studied. Maximum quantity of invertase (5.25 U/mL) was produced when the strain was grown on growth conditions (molasses, carbon source; yeast extract, nitrogen source; incubation time, 48 h; pH, 6.5; temperature, 35° C; inoculum size, 5x106 conidia; agitation rate, 150 rpm). The enzyme was also partially purified by salt precipitation and column chromatography. Temperature and pH optima were 55° C (106.8 U/mL) and 5.5 (121.7 U/mL), respectively. The strain was producing enzyme even up to pH 9.0 and 60° C, so it might be a useful strain for industrial utilization.
