Present investigation was the first of its type carried out on Pakistani population with respect to vitamin D receptor (VDR) gene polymorphism in Urinary tract infection (UTI) patients. Vitamin D, a secosteroid belongs to the nuclear receptor family. In urinary tract it produces its action by binding with vitamin D receptor in bladder epithelial cells inducing the expression of cathelicidin, a potent antimicrobial peptide. Polymorphism in VDR gene may results in lowering the activity of vitamin D that leads to the susceptibility of UTI. Here, we evaluated VDR Fok1 gene polymorphism with its correlation to the risk of UTI in Karachites. Genomic DNA through proteinase-K method was extracted using mouth epithelial cells from 31 infected and 62 controls of both genders. VDR Fok1 gene polymorphism was evaluated though PCR-RFLP. A non-significant (p=0.699) association of VDR Fok1 gene polymorphism among cases and control was observed, however risk may be observed for Ff genotype with an odds-ratio of 1.255 (95%CI=0.476-3.310). Moreover, in controls the gene was in Hardy Weinberg Equilibrium (p=0.859).
Mehr un Nisa Iqbal, Nida Fatima, Taseer Ahmed Khan, Ayaz Ahmed, Nazir Ahmed Lone. (2015) LACK OF ASSOCIATION BETWEEN VITAMIN D RECEPTOR FOK1 POLYMORPHISM AND URINARY TRACT INFECTION AMONG KARACHI POPULATION, , Volume 12, Issue 4.
