Abstract
Improvement of banana through conventional method have been very difficult due to
ploidy and sterility of most popular cultivars. Hence, given the difficulties in
conventional breeding, mutation breeding was attempted to induce variability and
obtain improved banana varieties. Therefore, banana cv. Tanduk shoots meristem (1.5
x 1.0 cm) were exposed to γ rays at doses ranging from 10-70 Gy. Subculture was
conducted up to four cycle followed by induction of rooting. The radio-sensitivity of
in vitro shoots towards radiation was assessed through the percentage of the explants
that survived the treatment. The highest survival rate (74%) among γ treated explants
recorded was in 10 Gy treatment and the lowest survivality (20%) was in 70 Gy. The
lethal dose (LD50) which had caused 50% mortality to the irradiated material was found
to be 37 Gy. The result on shoot growth showed that the highest shoot number (5) per
explant recorded at radiation dosage of 20 Gy, followed by 3 shoots per explant at a
dosage of 10 Gy and highest shoot length (3.43 cm) was observed at 10 Gy followed
by 20 Gy and 30 Gy treatment caused significant reduction in shoot length. Similarly,
10 Gy induced maximum root length, whereas explants irradiated with 20 Gy and 30
Gy caused the reduction in root length significantly. Gamma rays at the lower dosage
of 10 and 20 Gy imposed a significant impact on shoot growth and 30 Gy caused
reduction in growth. Hence, the present research was conducted to study the effect of
γ radiation on banana plant, while observing growth traits in γ irradiated explants.
Ferid Abdulhafiz, Fatimah Kayat, Suhana Zakaria. (2018) Gamma irradiation effect on the growth of Musa cv. Tanduk (AAB), Asian Journal of Agriculture and Biology, Volume 6, Issue 2.
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