The present work was aimed to study the effect of various grinding agents and detergents on the amount and purity of the extracted DNA from diverse plant samples. Agents supporting grinding process were liquid nitrogen, acid washed sand and glass beads, along with a number of concentrations of two detergents i.e. SDS (Sodium Dodecyl Sulphate) and CTAB (Cetyltrimethylammonium bromide). Six plants species Croton (Codiaeum variegatum), Tomato (Lycopersicon esculentum), Orchid (Orchis militaris), Date palm (Phoenix dactylifera L.), Pineapple (Ananas cosmosus) and Aloe (Aloe vera); were selected for DNA isolation and these plant species differ in their leaf texture as well as polysaccharides and phenolics content. The results obtained showed the presence of linear relationship between detergent and yield of the DNA with a little influence on the purity. The type of grinding agent did not significantly affect the purity of the extracted DNA but the amount of extracted DNA is greatly influenced. The purest DNA samples of all six plants species were selected and subjected to PCR amplification.
Saifullah Khan, Naheed Kauser, Hammad Afzal Kayani, Ameer Ahmed Mirbahar, Bushra Noman. (2015) EFFECT OF GRINDING AGENTS AND DETERGENTS ON THE QUALITY OF EXTRACTED DNA FROM DIVERSE PLANT SPECIES, , Volume 12, Issue 1.
