Abstract
Enzymatic hydrolysis has become outstanding technology in converting lignocellulosic
biomass to its xylose monomer for xylooligosaccharides (XOS) production. The
present work involves an investigation on the effects of enzyme loading, agitation
speed, substrate loading, temperature and hydrolysis time on enzymatic hydrolysis for
XOS production. Pretreated oil palm frond bagasse by dilute nitric acid was used for
enzymatic hydrolysis using Cellic HTec2. The effects of factors were analyzed by half
fractional factorial design 25-1 using Design Expert with Response Surface
Methodology (RSM) to achieve maximum XOS production. The results revealed that
the best enzymatic hydrolysis condition yielded 4.13 mg/L of XOS when conducted at
5% (w/v) of substrate loading, 50 U/mL enzyme loading with 200 rpm agitation speed
and 55°C for 4 hours of hydrolysis time. Two factors that contributed to the highest
production of XOS were substrate loading and enzyme loading. The model obtained in
this present research is significant with p-value < 0.0001 and R-squared of 0.9545. It is
recommended that model had a maximum point which is possible for the optimization
process later. Overall, the findings of this study suggest that Cellic HTec2 is a suitable
candidate for enzymatic hydrolysis of pretreated OPFB for higher XOS production.
Mat Yaacob, Nurul Aishah, Kamaliah Abdul Samad, Syed Mohd, Hafizuddin Wan, Jamaliah Jahim. (2019) Factors affecting enzymatic hydrolysis of oil palm frond bagasse using cellic HTec2 for xylooligosaccharides production, Asian Journal of Agriculture and Biology, Volume 7, Issue 1.
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