Cellulases are the collection of the enzyme mainly produced by the microorganisms. Recombinant strain of Thermotoga peterophila was used to produce the enzyme through submerged fermentation at 37°C for two days in latent broth (LB) as fermentation medium. The enzyme was purified by Ion exchange chromatography and activity was determined according to the procedure of Bernfeld. The maximum activity in enzyme entrapped in gel slices was observed at 900C when the enzyme was incubated for 2 hours and 30 minutes. For the enzyme entrapped in calcium alginate beads, maximum activity of the enzyme was observed at 1000C when the enzyme was incubated for 3 hours. No defined pH shift was observed in the enzyme entrapped in acrylamide gel slices whereas pH shift towards more acidic range for enzyme entrapped in calcium alginate beads. The shelf life of the enzyme, stored at 40C, 32 and 18 days enzyme entrapped in calcium alginate beads and acrylamide gel slices respectively.
