Abstract
This study reported on the optimization of enzymatic hydrolysis condition of edible
bird’s nest (EBN) using Protamex® to obtain maximum degree of hydrolysis degree
(DH). Besides, the proximate analyses of soaked cleaned raw EBN and its lyophilized
hydrolysate powder prepared under optimum condition (suggested in this study) were
also compared. Response surface methodology (RSM) was employed using a threelevel face-centered Central Composite Design (CCD) at four different parameters,
namely temperature (40-60oC), concentration of Protamex® (0.5-1.5%), pH (5.5-9.5)
and hydrolysis time (2-6 hr). It was found that a quadratic fit could explain the effect
of these four variables on the DH of EBN. The optimum condition was obtained at
temperature 59.9°C, pH of 6.3, Protamex® concentration of 2% and hydrolysis time of
5.4 hr. The DH achieved under this optimum condition (33.88%) was close to the
predicted DH (34.11%). It was found that the lyophilized EBN hydrolysate powder
prepared under the optimum condition gave similar protein and carbohydrate content,
but lower fat content and higher ash content as compared to cleaned raw EBN.
Mat Amin, Khuzma Din, Chow Hui. (2019) Optimization of enzymatic hydrolysis condition of edible bird’s nest using Protamex to obtain maximum degree of hydrolysis, Asian Journal of Agriculture and Biology, Volume 7, Issue 1.
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