Abstract
The reaction of 20 potato genotypes was determined using double antibody sandwich ELISA (DAS- ELISA) against six viruses viz; Potato Leaf Roll Virus (PLRV), Potato Virus X (PVX), Potato Virus Y (PVY), Potato Virus S (PVS), Potato Virus A (PVA) and Potato Virus M (PVM). The study was conducted at Potato Research Institute, Sahiwal during 2010-11 and 2011-12. The samples were collected from the field and tested in the laboratory of Plant Pathology at Potato Research Institute, Sahiwal. The reaction of ELISA for different viruses showed that out of 20 genotypes, different responses were detected with varying percentage against six potato viruses, some shows moderately susceptible behavior against potato viruses and some shows susceptible behavior: susceptible response means that there is not a single gene present which can resist against potato viruses. Five genotypes viz; FD 75-47, SH-5, FD 76-12, FD 74-33 and FD 74-8 showed the resistance response against all the potato viruses in both years experiments. This indicates that these 5 genotypes are the good source of resistance and may be useful for the development of potato cultivars resistant to potato viruses.

Sher Muhammad, Muhammad Shahbaz, Muhammad Tariq Javed, Muhammad Iqbal, Muhammad Shafiq Tariq, Muhammad Tasdiq Hussain Shahid, Nazar Hussain Khan. (2013) ELISA BASED SCREENING OF POTATO VARIETIES / LINES AGAINST POTATO VIRUSES, , Volume 10, Issue 4.
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