Abstract
An efficient callus induction protocol through nodes, internodes and leaves explants were established in Salvia santolinifolia, a medicinally important herb belonging to the family Lamiaceae. Calli were initiated on Murashige and Skoog (1962) (MS) medium supplemented with various concentrations of Napthalenacetic acid (NAA) (0.5, 1.0, 1.5, 2.0 and 2.5 mg/l) and Indole-3-acetic acid (IAA) (0.5, 1.0, 1.5, 2.0 and 2.5 mg/l) and BA (0.5). Maximum fresh weight (5.25 ± 2.39 g) of callus was achieved on MS medium containing 1.5 mg/l of NAA from leaf explants. It was observed that the combination of cytokinins and auxin suppressed callus induction.
