Abstract
Proteases are among the most important hydrolytic enzymes which have great potential
in various industrial processes such as leather, detergent, textile, food, feed industries.
Although many microorganisms produce these enzymes, in the recent period
Aspergillus fungi have most widely been used for proteases production. The production
of protease enzymes has been affected by a variety of physical and chemical factors,
such as inoculum concentration, time of incubation, pH, temperature, carbon, nitrogen
and mineral sources etc. However, composition of the cultivation medium (carbon and
nitrogen sources) play significant role in enzymes production. The aim of the present
study was the selection of suitable carbon and nitrogen sources of Aspergillus awamori
16 and Aspergillus awamori 22 mixed cultures for maximal production of extracellular
protease. Sucrose (4.2 U/ml) and peptone (4.8 U/ml) were found as the best carbon and
nitrogen sources, respectively.
Raushan Blieva, Nurlan Akhmetsa, Aigerim Zhakip, Aigul Kalieva, Zhanar Rakhme. (2019) Optimization of culture media for protease production by Aspergillus fungi, Asian Journal of Agriculture and Biology, Volume 7, Issue 2.
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