Abstract
The aim of this study was to develop an easy, fast, non-hazardous and inexpensive
technique for extraction of genomic DNA from multiple plant fungal pathogens.
Samples of pure fungal growth of Fusarium equesti , Neoscytalidium dimidiatum ,
Fusarium proliferatum and Alternaria alternata isolated from diseased wheat,
grapevine, potato and lily plants respectively were ground with sterilized sand and
NaOH (2N), followed by a centrifuging process to separate the sand grains and cellular
components of fungi from the DNA. Subsequently, the DNA was mixed with Tris
buffer (1 M) pH 8. The ITS region of rDNA was successfully amplified, sequenced and
analyzed from the extracted DNA of the four pathogenic fungi. This new approach
provides a simple, rapid, safe and low cost way to obtain DNA samples of sufficient
quantity and quality for use in molecular assays for the identification of plant fungi.
Adnan A. Lahuf, Ola H. Jaafar, Zainab L. Hameed. (2019) A simple, rapid, safe and low-cost method to extract DNA from phytopathogenic fungi, Asian Journal of Agriculture and Biology, Volume 7, Issue 2.
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