Biodiversity and biosystematics of free-living marine nematodes were investigated in water and sediment samples collected from five stations at Chinna Creek. Chinna Creek which is also known as Eastern Break water is about 5.0 km long , 300 to 500 m wide with a depth ranges from 1 to 3 meters and is situated at the South East of Karachi at Manora Channel. Faunastic survey of the benthic communities revealed a total of fifteen genera belonging to ten families in three orders from Chinna Creek. Epistrate feeders were the major trophic group at Chinna Creek, they comprised 46 % of the total nematode fauna followed by predators 38 % and deposit feeders 16 % only