Abstract
Five Oak species found in Pakistan are Q. baloot, Q. dilatata, Q. incana, Q. glauca and Q. semecarpifolia. The seed storage proteins in these five species of Oak found in Pakistan were analyzed. Proteins were extracted at room temperature for about 20 minutes in extraction buffer containing 0.05 M Tris, 2.0 % SDS, 5 M Urea, and 1-2% beta mercaptoethanol. Gels were prepared in 30% Acrylamide solution having Acrylamide: bisacrylamide ratio of 30:0.8. One sample of Quercus incana collected from Shawer; Swat was identified where a protein band was missing indicating possibilities of previously miss identification of the species. More research work is needed for better understanding of the genetic structure of the species.
