Abstract
This study aimed at the development of protocol for effective acclimatization of sugarcane plantlets (cv. SPC-79) regenerated through
tissue culture. After successful in vitro multiplication and rooting, sugarcane plantlets were transferred to the green house under
partially controlled conditions for acclimatization. To achieve maximum survival efficiency of in vitro regenerated plants, these were
transferred in soil medium supplied with various types of nutrient solutions i.e. ½ MS salts, 10 % coconut water, 1 % DAP + 1 %
urea in 1:1 (w/w) and 5 % farm yard manure. It was found that among all the nutrient solutions tested during acclimatization,
application of ½ MS salts solution proved to be the most effective treatment closely followed by 10 % coconut water, for achieving
significant survival efficiency by improving the growth characteristics of in vitro grown sugarcane plants.
