Date syrup is a liquid waste of date processing industries. It is rich in reducing and non reducing sugars and
may be used as a fermentation substrate. Cotton stalk is rich in cellulose and hemi cellulose. Pretreated
(Physical and chemical) cotton stalk is also used as a carbon source for the production and optimization of
industrial enzyme, Invertase (β-fructofuranosidase, E.C.3.2.1.26). Different nitrogen sources (corn-steep,
casein, peptone potassium nitrate etc.) different pH (4-9) and temperatures (20-55) have been used. In the
present study an indigenous fungus Penicilium Lilacinum is used for enzyme production. It was found that
invertase production was maximum after 72 hours (on 0.6 N H2SO4 treated cotton stalk) and 96 hours (on
5% date syrup), production is enhanced by peptone as a nitrogen source at pH 8 and at a temperature of 40
оC
Kashif Ahmed, M. Umer Dahot, Qamar-ul-Haq, Ehsan Elahi Valeem. (2011) OPTIMAL CONDITIONS FOR THE PRODUCTION OF COMMERCIAL ENZYME BY PENICILIUM LILACINUM BY CULTURING ON AGRO INDUSTRIAL WASTE, , Volume 08, Issue 2.
